![]() Various sources of evidence were used to predict the association between proteins such as curated databases (light blue), experimentally determined (pink), gene neighbourhood (green), gene fusion (red), gene co-occurrence (dark blue), textmining (yellow) and co-expression (black). The numbers of lines are directly proportional to the strength of prediction for the functional association between proteins. Each node represents an individual protein and the interconnecting lines display the predicted functional interaction between the connected protein nodes. The protein-protein interaction network of DEAS genes in brown trout during PKD. Myxozoan Post-transcriptional modification RNA-seq Salmonids Tetracapsuloides bryosalmonae. The information generated from the present study can help to develop therapeutic strategies for PKD in the future. The generated data lay a foundation for further functional molecular studies in PKD - brown trout infection model. This study provides the first baseline information about alternative splicing in brown trout during PKD. Furthermore, the human disease-related salmonella infection pathway was significantly enriched in the protein-protein interaction network. The protein-protein interaction network analysis enriched two local network clusters namely cation transporting ATPase C-terminus and Sodium/potassium ATPase beta chain cluster, and mixed inclusion of Ion homeostasis and EF-hand domain cluster. The DEAS genes were significantly enriched for sodium-potassium transporter activity and ion homeostasis (ahcyl1, atp1a3a, atp1a1a.1, and atp1a1a.5). Among the DEAS genes, the least and most abundant alternative splicing types were alternative 5' splice site (5.23%) and exon skipping (70.59%), respectively. About 153 significant differently expressed alternatively spliced (DEAS) genes, (delta PSI = 5%, FDR P-value < 0.05) were identified from 19,722 alternatively spliced events. Subsequently, this data was mapped to the brown trout genome. RNA-seq data were generated from the posterior kidney of brown trout collected at 12 weeks post-exposure to T. We investigated the alternative splicing pattern in the posterior kidney of brown trout during PKD. bryosalmonae undergoes intra-luminal sporogonic development in the kidney of brown trout (Salmo trutta) and the viable spores are released via urine. This parasite is a serious threat to wild and cultured salmonids. The cnidarian myxozoan parasite Tetracapsuloides bryosalmonae causes chronic proliferative kidney disease (PKD) in salmonids.
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